http://www.fordycelab.com/portfolio daily 1.0 2020-12-10 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1443674181824-FA3WGIHSIQ64PW8XOB5K/Lanthanides-1.jpg Home https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1443674181824-FA3WGIHSIQ64PW8XOB5K/Lanthanides-1.jpg Home https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1403651812933-U03ZWJ0FB6MWE1PXI3TJ/devicefromcorner_marshall.jpg Home https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1403651985401-ZAXV8HGTFMD07T27S27F/microfluidics-4859.jpg Home https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1403908760076-645QP7GY1XLJ75SCR58R/microfluidics-4852.jpg Home https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1443674235940-ST8UQFA6PFNBTDU4WVSC/DeviceOnScopeLitUp.jpg Home https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1443674375878-R01KQ2KD3HK80X8GAFMY/DeviceOnLightBoxFromTheSide.jpg Home https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1607579346689-ZYD39DKOXKIJBTW0X00L/pasted-image.jpg Home http://www.fordycelab.com/blog daily 0.75 2025-03-20 http://www.fordycelab.com/blog/2025/3/20/check-out-our-latest-pre-print monthly 0.5 2025-03-20 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/7f849f0e-b343-4a58-8f10-5a82050f4442/Thompson_preprint.jpg News - Check out our latest pre-print! If you are interested in high-throughput microfluidic droplet screening, check out our latest pre-print “Continuous FACS sorting of double emulsion picoreactors with a 3D printed vertical mixer.“ This project was led by postdocs Samuel Thompson & Zijian (ZJ) Yang's (from the Soh lab) and demonstrates successful FACS sorting double emulsions at rates of 1,000,000/hour! http://www.fordycelab.com/blog/2021/9/14/welcome-gaeun-a88a7 monthly 0.5 2025-03-03 http://www.fordycelab.com/blog/2024/6/5/congratulations-nicole monthly 0.5 2024-06-05 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/310a4b8d-dbdb-416f-a0d8-70087fafe423/image+%28108%29.png News - CONGRATULATIONS NICOLE! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. http://www.fordycelab.com/blog/2024/6/4/congratulations-polly monthly 0.5 2024-06-04 http://www.fordycelab.com/blog/2024/6/4/congratulations-matt monthly 0.5 2024-06-04 http://www.fordycelab.com/blog/2024/6/4/congratulations-eliel-1 monthly 0.5 2024-06-04 http://www.fordycelab.com/blog/2021/10/14/welcome-pierre-emmanuel-thiriet monthly 0.5 2021-10-15 http://www.fordycelab.com/blog/2021/9/21/welcome-jennifer monthly 0.5 2021-09-21 http://www.fordycelab.com/blog/2021/9/14/welcome-gaeun monthly 0.5 2021-09-14 http://www.fordycelab.com/blog/2021/9/1/welcome-daria monthly 0.5 2021-09-01 http://www.fordycelab.com/blog/2021/4/8/welcome-micah monthly 0.5 2021-04-08 http://www.fordycelab.com/blog/2021/4/2/welcome-minsung monthly 0.5 2021-04-02 http://www.fordycelab.com/blog/2021/3/23/congratulations-michael monthly 0.5 2021-03-24 http://www.fordycelab.com/blog/2021/3/1/welcome-to-the-world-rex monthly 0.5 2021-03-01 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1614614105679-4NPL3WNJ6NV8KW8VIFIR/PXL_20210208_221712854.PORTRAIT-1.jpg News - Welcome to the world, Rex! http://www.fordycelab.com/blog/2021/2/19/congratulations-conor monthly 0.5 2021-02-19 http://www.fordycelab.com/blog/2021/1/20/welcome-julia monthly 0.5 2021-01-20 http://www.fordycelab.com/blog/2020/9/22/congratulations-peter monthly 0.5 2020-09-22 http://www.fordycelab.com/blog/2020/9/22/congratulations-to-andy-and-the-beads-team monthly 0.5 2020-09-22 http://www.fordycelab.com/blog/2020/9/22/welcome-renee monthly 0.5 2020-09-22 http://www.fordycelab.com/blog/2020/9/15/farewell-to-gauen-and-jamin monthly 0.5 2020-09-15 http://www.fordycelab.com/blog/2020/9/15/welcome-samuel monthly 0.5 2020-09-15 http://www.fordycelab.com/blog/2020/9/15/congrats-bea monthly 0.5 2020-09-15 http://www.fordycelab.com/blog/2020/9/15/welcome-michael monthly 0.5 2020-09-15 http://www.fordycelab.com/blog/2020/6/30/arjun-aditham-publishes-a-paper monthly 0.5 2020-07-02 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1593714423912-LCIW13F0FPOCWWN2J672/image-asset.png News - Arjun Aditham publishes a paper! http://www.fordycelab.com/blog/2020/6/30/andy-publishes-paper monthly 0.5 2020-06-30 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1593543077670-4LT3MR1GWBA3XKNVZ6QS/Screen+Shot+2020-06-30+at+11.48.06+AM.png News - Congratulations to Yinnian (Andy) Feng on his new manuscript! http://www.fordycelab.com/blog/2020/6/30/kara-brower monthly 0.5 2020-07-02 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1593714108811-8R4QOLJJX57JCSJ1BCTG/Screen+Shot+2020-06-30+at+10.17.43+AM.png News - Kara Brower publishes paper! http://www.fordycelab.com/blog/2020/5/29/nicole-delrosso-passes-her-quals monthly 0.5 2020-05-29 http://www.fordycelab.com/blog/2019/10/2/fordyce-lab-tours-our-new-lab-space monthly 0.5 2019-10-03 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1570068574189-DKL4WCE781FI297R1V0A/IMG_6137.JPG News - Fordyce Lab Tours Our New Lab Space! http://www.fordycelab.com/blog/2019/4/19/polly-is-the-speaker-of-lets-have-an-awesome-time-doing-science-may-1-2019 monthly 0.5 2019-04-19 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1555713019397-4QLFYMBJVWAZUA1VHVTJ/LHAATDS_flyer_2019.jpg News - Polly is the speaker of "Let's Have an Awesome Time Doing Science" - May 1, 2019 http://www.fordycelab.com/blog/2019/4/12/congratulate-gaeun-for-receiving-75k-on-an-reu-fellowship monthly 0.5 2019-04-12 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1555111038553-O8ZALICSHNQVBO6SF4ZH/image-2.jpg News - congratulate Gaeun for receiving $7.5k on an REU Fellowship! http://www.fordycelab.com/blog/2017/8/31/polly-named-scialog-fellow-kdddg monthly 0.5 2019-04-12 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1555096090931-DZDUR6GFM7Z9NKKZZIFU/birthday.jpg News - Hooray! Happy Birthday to Adam and Mason! https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1555096175937-MWO4S20XWMV904RMPFXK/birthday%2B2.jpg News - Hooray! Happy Birthday to Adam and Mason! http://www.fordycelab.com/blog/2019/3/14/professor-polly-fordyce-to-give-her-vision-talk-this-friday monthly 0.5 2019-04-12 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1555110567024-TDXKKB6CVMIWUS7YDGBF/Capture.jpg News - Professor Polly Fordyce to give her Vision Talk this Friday http://www.fordycelab.com/blog/2019/4/12/craig-and-daniels-poster-at-biophysical-society-conference-was-popular monthly 0.5 2019-04-12 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1555111444955-7UO1UTZIS6CA7YN3YZEJ/Image%2Bfrom%2BiOS%2B%25281%2529.jpg News - Craig and Daniel’s Poster at Biophysical Society Conference was popular. http://www.fordycelab.com/blog/2017/8/31/polly-named-scialog-fellow monthly 0.5 2019-04-02 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1504211714306-X7Z0IPA9J9Q0JQP1JHTZ/rescorp_logo.png News - Polly named Scialog Fellow https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1504212053925-KUP7TH0XV7JBP1I7FXCW/gordon+betty+moore.jpeg News - Polly named Scialog Fellow http://www.fordycelab.com/blog/2017/8/31/bianca-cruz-joins-the-lab monthly 0.5 2017-09-01 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1504211381487-EXNIFAS9L7LHBA6EP78Z/bianca+cruz.png News - Bianca Cruz joins the lab for the summer! http://www.fordycelab.com/blog/2018/9/5/kara-and-craig-present-at-the-gordon-research-conference monthly 0.5 2018-09-06 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1536215033377-MQCRF2ZVHMFA8TDFG51L/IMG_8462.jpg News - Polly, Kara & Craig attend the GRC Microfluidic Conference in Italy https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1536215313631-7VALAG75MTMWYV8IBZM6/IMG_8469.jpg News - Polly, Kara & Craig attend the GRC Microfluidic Conference in Italy http://www.fordycelab.com/blog/2017/8/13/karas-paper-describing-an-open-source-pneumatics-build-for-microfluidic-control-is-posted-in-biorxiv monthly 0.5 2019-04-02 http://www.fordycelab.com/blog/2017/6/6/kara-earns-a-centennial-teaching-award monthly 0.5 2019-04-02 http://www.fordycelab.com/blog/2017/5/10/pollys-microfluidics-work-covered-in-stanford-alumni-1 monthly 0.5 2017-05-10 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1494457911490-OF52RQTEMDTPX71ZDSFV/image-asset.jpeg News - Polly's course covered in Stanford Alumni Magazine! http://www.fordycelab.com/blog/2017/3/9/polly-in-the-news monthly 0.5 2017-03-10 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1489114383465-GOL631DGYIQP4RMT6Y93/image-asset.jpeg News - Polly profiled for Stanford Medicine News http://www.fordycelab.com/blog/2017/2/27/alli-keyes-joins-the-lab monthly 0.5 2017-03-10 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1488225506912-45VIPPN0WTXRS3EBEN0O/image-asset.png News - Alli Keys joins the lab! http://www.fordycelab.com/blog/2017/2/23/polly-is-named-a-2017-sloan-fellow monthly 0.5 2017-02-24 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1487894496354-HM62JNKRDDTA099EU6X8/image-asset.jpeg News - Polly is named a 2017 Sloan Fellow! http://www.fordycelab.com/blog/2017/2/21/polly-is-named-a-chan-zuckerberg-investigator monthly 0.5 2017-02-22 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1487743554539-71TQTNT5F0W0GHG8M3PT/image-asset.png News - Polly is named a Chan Zuckerberg Investigator! http://www.fordycelab.com/blog/2017/2/21/alex-sockell-joins-the-lab monthly 0.5 2017-02-22 http://www.fordycelab.com/blog/2016/11/29/film-crew-visits-the-lab-for-our-upcoming-jove-article monthly 0.5 2016-11-30 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1480466672656-WXDEB6ES5RQM1U1RCDNJ/File_000.jpeg News - Film crew visits the lab for our upcoming JoVE article! https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1480466672749-OQFC322A9UMTCOE81G61/File_001.jpeg News - Film crew visits the lab for our upcoming JoVE article! https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1480466673856-PUO9ZBZIIZKT2EMCZH12/File_002.jpeg News - Film crew visits the lab for our upcoming JoVE article! https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1480466674803-9A4LJBMJ7T47REU6UK1B/File_003.jpeg News - Film crew visits the lab for our upcoming JoVE article! http://www.fordycelab.com/blog/2016/11/29/tyler-officially-becomes-a-phd-candidate monthly 0.5 2016-11-30 http://www.fordycelab.com/blog/2016/10/18/our-new-paper-describing-spectrally-encoded-beads-with-1000-codes-is-out monthly 0.5 2016-10-18 http://www.fordycelab.com/blog/2016/10/4/the-fordyce-lab-is-awarded-an-nih-new-innovator-award monthly 0.5 2016-10-04 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1475620525131-3V8Q98YQIODU6WKH1N3L/image-asset.jpeg News - The Fordyce Lab is awarded an NIH New Innovator Award! http://www.fordycelab.com/blog/2016/9/27/the-fordyce-lab-is-awarded-2-bio-x-seed-grants monthly 0.5 2016-09-28 http://www.fordycelab.com/blog/2016/7/27/welcome-huy-quoc-nguyen-and-congratulations monthly 0.5 2016-07-27 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1469647163543-A3GMMCYY3OCATV13K9KT/image-asset.jpeg News - Welcome Huy Quoc Nguyen and congratulations! http://www.fordycelab.com/blog/2016/7/6/welcome-rebecca-varun-and-alex monthly 0.5 2016-07-06 http://www.fordycelab.com/blog/2018/9/5/craig-is-awarded-a-cihr-postdoctoral-fellowship monthly 0.5 2018-09-06 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1536211815852-QU01POYSORTFCOF8YOMR/1200px-Canadian_Institutes_of_Health_Research_Logo.svg.png News - Craig is awarded a CIHR Postdoctoral Fellowship! http://www.fordycelab.com/blog/2016/6/22/adam-white-is-awarded-a-stanford-deans-postdoctoral-fellowship monthly 0.5 2016-06-23 http://www.fordycelab.com/blog/2016/6/22/welcome-arjun-aditham-and-scott-longwell monthly 0.5 2016-06-23 http://www.fordycelab.com/blog/2016/6/16/fordyce-lab-wins-a-nist-jimb-seed-grant monthly 0.5 2016-06-17 http://www.fordycelab.com/blog/2016/6/13/welcome-tyler-shimko monthly 0.5 2016-06-13 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1465843462184-GMXOCPACOZIEXSZF0BXQ/image-asset.jpeg News - Welcome Tyler Shimko! http://www.fordycelab.com/blog/2016/4/18/bioengineering-retreat-2016 monthly 0.5 2016-04-18 http://www.fordycelab.com/blog/2016/4/18/fordyce-lab-awarded-a-beckman-technology-development-grant monthly 0.5 2016-04-18 http://www.fordycelab.com/blog/2016/2/18/welcome-to-tyler-dave-and-scott-1 monthly 0.5 2016-02-19 http://www.fordycelab.com/blog/2015/10/1/welcome-linfeng-and-theo monthly 0.5 2015-10-02 http://www.fordycelab.com/blog/2015/8/5/congratulations-to-kara-on-her-seed-grant monthly 0.5 2015-08-06 http://www.fordycelab.com/blog/2015/8/5/welcome-craig-markin-1 monthly 0.5 2015-08-06 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1438832950460-9143BTTNIXIVFYHVM739/FordyceLabOnTheCouch.jpg News - Welcome Craig Markin! http://www.fordycelab.com/blog/2015/6/30/welcome-chantal-guegler monthly 0.5 2015-07-01 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1435711062557-7THHCSRBEBZVCYGF4ZIR/image-asset.jpeg News - Welcome Chantal Guegler! http://www.fordycelab.com/blog/2015/5/14/seeking-applicants-for-joint-postdoctoral-position-with-novartis-and-nistabms monthly 0.5 2015-05-15 http://www.fordycelab.com/blog/2015/4/20/kara-presents-the-first-fordyce-lab-poster monthly 0.5 2015-04-21 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1429595949392-ER2GFGLGSABI9044K8K1/KaraPoster_small.jpg News - Kara presents the first Fordyce Lab poster! http://www.fordycelab.com/blog/2015/3/31/congratulations-to-kara-eli-and-naomi monthly 0.5 2015-03-31 http://www.fordycelab.com/blog/2015/3/31/welcome-to-naomi-and-chris monthly 0.5 2015-04-20 http://www.fordycelab.com/blog/2015/2/24/kara-brower-joins-the-fordyce-lab monthly 0.5 2015-04-20 http://www.fordycelab.com/blog/2015/2/24/more-lab-firsts monthly 0.5 2015-02-25 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1424841273077-HQVQBBYQG4N0HF6ASDUW/image-asset.jpeg News - More lab firsts! https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1424841491530-PRM06Q2IT1MDKEFTKB45/image-asset.jpeg News - More lab firsts! http://www.fordycelab.com/blog/2015/1/23/a-series-of-lab-firsts monthly 0.5 2015-01-23 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1422031498560-PMSFC3RL039973YDBX3C/image-asset.png News - A series of lab firsts First lab gel! https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1422031612335-MPUV7MSI38Q9Y4V50OLP/image-asset.jpeg News - A series of lab firsts First wafer fabrication! http://www.fordycelab.com/blog/2014/11/12/robert-puccinelli-joins-the-fordyce-lab monthly 0.5 2014-11-13 http://www.fordycelab.com/blog/2014/6/27/fordyce-lab-opens-for-business-at-stanford monthly 0.5 2014-06-30 http://www.fordycelab.com/people-gallery daily 0.75 2026-01-13 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1700010920630-VCCZLI9K1XNIRIKXWYDA/IMG_4529.jpg People Gallery - Polly Fordyce Principal Investigator. She/Her Click here for CV. Click here for bio. Hobbies: Running, climbing, hiking, and spending time with my family https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1700010920630-VCCZLI9K1XNIRIKXWYDA/IMG_4529.jpg People Gallery - Polly Fordyce Principal Investigator. She/Her Click here for CV. Click here for bio. Hobbies: Running, climbing, hiking, and spending time with my family https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1671768030215-LGFSQFV7X1GKQH34XL3Y/almhjell_headshot_Original.jpg People Gallery - Patrick Almhjell Postdoctoral Fellow (joint with Herschlag Lab) He/Him Google Scholar Hobbies: Snowboarding, climbing, general adventuring https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1690322019112-HBPDVQHRE9IMG5H6MSTL/Eliel_headshot.jpeg People Gallery - Eliel Akinbami Graduate Student Bioengineering He/Him Hobbies: Baking, cooking, olympic weightlifting, drumming https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1741026095023-VWW1VHGJYS0VO4TL98KY/image-asset.jpeg People Gallery - Yujia Bian Graduate Student Chemical Engineering She/They Hobbies: Baking, cooking, painting, swimming, playing computer games and ttrpg, learning fun facts about octopus. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1690309784205-I3AB1D615JU3VOA3O3F9/image-asset.jpg People Gallery - Minsung Cho Graduate Student Structural Biology He/Him Hobbies: Rock climbing, video games, eating food https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1700010920630-VCCZLI9K1XNIRIKXWYDA/office-wall.jpg People Gallery - Polly Fordyce Principal Investigator. She/Her Click here for CV. Click here for bio. Hobbies: Running, climbing, hiking, and spending time with my family https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1763498880108-FDE7LDCR9RXCI49JIJT0/image-asset.jpeg People Gallery - Carlos Cortez Postdoctoral Fellow Genetics He/Him Hobbies: Music, animals, plants, cooking, and being outside. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1690305793215-MV7DNE1JP09D7TZIQUUD/SMC_headshot+-+Shawn+Costello.png People Gallery - Shawn Costello Postdoctoral Fellow (joint with Herschlag Lab) He/Him Hobbies: Reading, crafting, rewatching The West Wing https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1724890357898-2WWIJFJUYGTFLZYBVD84/Matt_head_shot.jpeg People Gallery - Matt DeJong Graduate Student Chemical Engineering He/Him Hobbies: Running, hiking, tossing frisbee, and solving the Monday NYT crossword puzzle https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1763488260795-JRB4EY57CGA7UBCWUPVP/image-asset.jpeg People Gallery - Chase Freschlin Postdoctoral Fellow Genetics Hobbies: biking, baking, sewing, thinking about stories. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1690307766672-XY3AGXF0V9VGYJ5RGDMK/spruce4006_grad41.jpg People Gallery - Renee Hastings Graduate Student Biophysics She/Her Hobbies: Backpacking, skiing, painting, snarking on reality TV, and board gaming https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1536714000452-WYE44BFNKPHZ9JLWL2V2/RSD17_049_0977a.jpg People Gallery - Polly Fordyce Principal Investigator. Click here for CV. Click here for bio. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1601443411889-ARO9HODTGZKAZIYBIGYH/IMG_20190910_112329%252B%2525282%252529.jpg People Gallery - Michael Hayes Graduate Student Genetics He/Him Hobbies: Drumming, hiking with my dog, cooking https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1677122306848-6IAMQHGHUHV5187Q5OSR/IMG_2975.JPG People Gallery - Caroline Horn Fordyce Lab Manager She/Her Hobbies: Hiking, biking, and accumulating library cards all around the Bay Area https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1690309707933-48Y45512BD6MX9K2VD8M/IMG-0628.jpg People Gallery - Devyn James Administrative Assistant Bioengineering She/Her https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1695152697714-MOB49SAXC112GA52Y699/JessicaKaraguesianHeadshot%2B-%2BJessica%2BKaraguesian.jpg People Gallery - Jessica Karaguesian Graduate Student Bioengineering (Joint w/Dror Lab) she/her Hobbies: Hiking, skiing, eating any and all sweets https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1671768195503-2G8KOSSUUX1N8DT8MD4L/received_425564376128394.jpg People Gallery - Karl Krauth Postdoctoral Fellow He/Him Personal Site Hobbies: charge they phone, eat hot chip & lie https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1724784328190-05OFC87T9KFNIMK5MYQO/Micah+Lawrence_Feb_2023_01.jpg People Gallery - Micah Lawrence Graduate Student Bioengineering He/Him Hobbies: Watching anime, playing basketball and volleyball, and live music https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1671768149332-70TCM5Q3GIAA6MZVIKIE/IMG_0213.jpg People Gallery - Albert Lee Postdoctoral Fellow (joint with Herschlag lab) He/Him Hobbies: Rock climbing, baseball, snowboarding https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1690304153989-2P9SK7QWLX1A3MFI6MH1/image-asset.jpg People Gallery - Byungjin Lee Postdoctoral Fellow Genetics He/Him Hobbies: Cooking https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1690306227215-PMO5Y8KJ3BQSLIQLSTAW/me+-+Youngbin+Lim.jpeg People Gallery - Youngbin Lim Senior Research Technician LinkedIn Hobbies: Playing golf, going to the gym, and trying new hamburgers https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1755638304920-MIDQRPE1X59B76KVKULY/LMNewNew.png People Gallery - Lucas Melo Gradute Student Genetics He/Him Hobbies: tea, maps, Only Connect, Canadian constitutional law https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1690304697814-MM3C1NPENVC25GVM6784/IMG_0268.jpg People Gallery - Conor McClune Postdoctoral Fellow (joint with Sattely Lab) He/Him Hobbies: Trail running, gardening, sparring with the HOA https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1732563806416-MVCVJVUGNB5Q6JEQI5Y9/foto+perfil.jpg People Gallery - Ana Morzan Lab Administrator Hobbies: Running, backpacking, climbing, and crocheting https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1719347030632-W5TRA1721AUZN0NRHHEA/IMG_0066.jpeg People Gallery - Micah Olivas Graduate Student Genetics He/Him Personal Site Hobbies: Live music, cooking, playing guitar, crashing weddings https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1633110809590-WM8C4B7UKCJVOO5YYUOF/Screen+Shot+2021-10-01+at+10.52.42+AM.png People Gallery - Jennifer Ortiz Cárdenas Microfluidics Foundry Director & Outreach Coordinator She\Her Hobbies: Discovering new restaurants and wine bars, taking my dogs to the beach in Half Moon Bay, cooking new recipes, and watching reality TV. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1724890300226-OGNUDCL7IEH8O7EU6F2P/image-asset.jpeg People Gallery - Daria Passow Graduate Student Biophysics She/Her Hobbies: listening to audiobooks, walking and cycling, reading MotorTrend magazine https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1755638326650-8BI15PF0T1N3LLD4F6WP/2025_professional_headshot_lowres.jpg People Gallery - Lillian Petersen Graduate Student Genetics (joint with Bintu Lab) Hobbies: Running, hiking, cycling, skiing, reading, violin, exploring art museums https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1690305635891-X0A7NSLUMIDTP7PO1ZXW/IMG_7644+-+Maya+Sheth.jpeg People Gallery - Maya Sheth Graduate Student Bioengineering She/Her Hobbies: Hiking, dogs, crossword puzzles https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1724890412813-QG1LMVUIIS8Y1S2FQT3W/F73BE9BB-58AB-4647-8989-23B728F5AAFD_1_105_c.jpeg People Gallery - Alan Su Graduate Student (joint w/ Petrov Lab) Chemical & Systems Biology He/Him Hobbies: Basking in the glorious sun, squash, Formula 1, MTV's The Challenge, soup. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1536714108204-IQ2E6J446TPT9269JOHM/office-wall.jpg People Gallery - Polly Fordyce Principal Investigator. Click here for CV. Click here for bio. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1690309760758-8UOS46JQ8C8538BMZLQF/photo.jpg People Gallery - Peter Suzuki Graduate Student (joint with Bintu Lab) Bioengineering He/Him (LinkedIn) Hobbies: Music of all sorts (guitar + violin, from Silvio Rodriguez to the Strokes), baseball, policy/politics podcasts https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1415832767158-N1DL6IWPZTPDPXCJCUY2/RobertPuccinelli_2p5inchestall.jpg People Gallery - Robert Puccinelli Research Associate. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1690305994248-Y707FEBJ62M19G4Q536I/23_crop.jpg People Gallery - Samuel (Channel) Thompson Postdoctoral Fellow (joint with Baker Lab) He/She/They LinkedIn Hobbies: Baking, drawing, writing, listening to lots of audiobooks http://www.fordycelab.com/research-summary-gallery daily 0.75 2019-11-20 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474958399717-T0OY8IZ0MA8NSFMOCSA0/devicefromend_wlightbox_01_cropped_SMALL.jpg Research Summary Gallery MITOMI (Mechanically-Induced Trapping of Molecular Interactions) relies on spatial multiplexing to keep track of analytes throughout an experiment. We are currently using these devices to better understand how transcription factors find and bind their genomic targets to regulate gene expression, as well as to understand how enzymes achieve their extraordinary catalytic efficiency and substrate specificity. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474958399717-T0OY8IZ0MA8NSFMOCSA0/devicefromend_wlightbox_01_cropped_SMALL.jpg Research Summary Gallery MITOMI (Mechanically-Induced Trapping of Molecular Interactions) relies on spatial multiplexing to keep track of analytes throughout an experiment. We are currently using these devices to better understand how transcription factors find and bind their genomic targets to regulate gene expression, as well as to understand how enzymes achieve their extraordinary catalytic efficiency and substrate specificity. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474958323594-MD9YVQSRCZGTG18BZUYK/CoverArt_Option2.jpg Research Summary Gallery MRBLEs (Microspheres with Ratiometric Barcode Lanthanide Encoding) rely on spectral multiplexing to track analytes throughout an experiment. In these assays, we can create microspheres containing > 1,000 unique ratios of lanthanide nanophosphors that can be uniquely identified via imaging alone. We are currently developing new assays that use these microspheres to understand how signaling proteins recognize their peptide substrates and to improve our ability to extract information from single cells. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474913093720-O4IEAAV2C1P5E6E3XG2P/devicefromend_wlightbox_01_cropped_SMALL.jpg Research Summary Gallery - MITOMI MITOMI (Mechanically-Induced Trapping of Molecular Interactions) relies on spatial multiplexing to keep track of analytes throughout an experiment. We are currently using these devices to better understand how transcription factors find and bind their genomic targets to regulate gene expression, as well as to understand how enzymes achieve their extraordinary catalytic efficiency and substrate specificity. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474958296928-MMGXAY22CY9BP5SG654J/devicefromend_wlightbox_01_cropped_SMALL.jpg Research Summary Gallery MITOMI (Mechanically-Induced Trapping of Molecular Interactions) relies on spatial multiplexing to keep track of analytes throughout an experiment. We are currently using these devices to better understand how transcription factors find and bind their genomic targets to regulate gene expression, as well as to understand how enzymes achieve their extraordinary catalytic efficiency and substrate specificity. http://www.fordycelab.com/publications-gallery daily 0.75 2016-11-04 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1475094955982-1LYKCV0AVE9M3O7P38AX/IconForWebsite.png Publications Gallery 2016 Multi-step variable height photolithography for valved multilayer microfluidic devices. Brower, K.*, White, A.K.*, & Fordyce, P.M. Journal of Visualized Experiments (in press). (* denotes equal authorship). https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1475094955982-1LYKCV0AVE9M3O7P38AX/IconForWebsite.png Publications Gallery 2016 Multi-step variable height photolithography for valved multilayer microfluidic devices. Brower, K.*, White, A.K.*, & Fordyce, P.M. Journal of Visualized Experiments (in press). (* denotes equal authorship). https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474961534820-B8VTGPUFQU6VFWP7PFD9/CoverArt_Option2.jpg Publications Gallery 2016 Programmable microfluidic synthesis of over one thousand uniquely identifiable spectral codes. Nguyen, H.Q., Baxter, B.C., Brower, K., Diaz-Botia, C.A., DeRisi, J.L, Fordyce, P.M.*, and Thorn., K.S.* Advanced Optical Materials (2016). (* denotes co-corresponding authors). (pdf) (web) http://www.fordycelab.com/research-project-gallery daily 0.75 2016-09-27 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474958833280-37FBCEJCLQBJMDE4AUML/160919_MITOMI_DNA_Bound.jpg MITOMI Project Gallery Probing how transcription factors find and bind their genomic targets Gene expression is governed by binding of transcription factor proteins to regulatory sequences in the genome, where they either recruit or block transcriptional machinery to activate or repress expression. Despite many years of research, we remain largely unable to predict where transcription factors bind in vivo, and current models cannot explain how closely related transcription factors often regulate very distinct sets of target genes. We are combining the MITOMI platform with tools from chemical biology to precisely dissect the biophysical mechanisms by which transcription factors recognize DNA and improve our ability to predict these essential interactions.   https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474958833280-37FBCEJCLQBJMDE4AUML/160919_MITOMI_DNA_Bound.jpg MITOMI Project Gallery Probing how transcription factors find and bind their genomic targets Gene expression is governed by binding of transcription factor proteins to regulatory sequences in the genome, where they either recruit or block transcriptional machinery to activate or repress expression. Despite many years of research, we remain largely unable to predict where transcription factors bind in vivo, and current models cannot explain how closely related transcription factors often regulate very distinct sets of target genes. We are combining the MITOMI platform with tools from chemical biology to precisely dissect the biophysical mechanisms by which transcription factors recognize DNA and improve our ability to predict these essential interactions.   https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474958883719-LGMHY71MT9RGT2MT4KY3/Rplot.png MITOMI Project Gallery Revealing how kinetics of transcription factor binding affect occupancy and gene expression Recent evidence suggests that non-equilibrium binding dynamics may govern transcription factor activity at regulatory sites in the genome. Despite this fact, nearly all investigations of transcription factor binding in vitro measure only binding at equilibrium, without observing kinetics. We are developing a high-throughput microfluidic assay that can measure the residency time of molecular interactions. We expect this assay will be compatible with both extracted DNA and chromatin, allowing for investigations of the native epigenetic contexts that shape gene expression. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474958855217-QSDIVBY5GCOQH2M2H4S4/craig_AP_network_figure.jpg MITOMI Project Gallery Understanding how enzymes achieve their extraordinary catalytic efficiency and specificity Enzymes are the most efficient catalysts known, enhancing specific reaction rates by up to 17 orders of magnitude. However, we do not fully understand how they achieve this tremendous catalytic efficiency, which limits our ability to understand their biological function and design new enzymes with industrially or medically important applications. In close collaboration with the Herschlag lab, we are using the MITOMI platform to enable high-throughput investigation of how individual residues throughout an enzyme contribute to catalysis. This new platform allows detailed biochemical characterization of thousands of enzymes in parallel, enabling structure/function studies of enzymes at unprecedented scale.   https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1475004169196-NY8C3JG8B5UC5KJ8E0L4/IMG_20160927_113332.jpg MITOMI Project Gallery Automating printing and image acquisition and analysis Uncovering the biophysical mechanisms that drive transcription factor binding requires the ability to efficiently and reproducibly collect and compare data from many proteins. To streamline this process, we have been automating all asepcts of the experiments, from rebuilding old microarray printers to developing new software for valve automation and image processing. http://www.fordycelab.com/mrbles-project-gallery daily 0.75 2016-09-27 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474960907065-PUCQH7LN3UVEVFQSBY4T/PxIxIT+Library+2+CN+WT+250+nM.jpg MRBLEs Project Gallery A high-throughput assay for profiling protein-peptide interactions While advances in deep sequencing have dramatically improved our ability to profile how DNA and RNA binding proteins interact with their substrates, we lack similar tools for quantitative, high-throughput investigation of protein-peptide interactions. We have recently demonstrated that peptides can be synthesized directly on our encoded MRBLEs, laying the foundation for novel high-throughput on-bead assays to probe how proteins recognize their peptide substrates. In collaboration with the Cyert Lab, we are using this platform to understand how calcineurin, a human phosphatase essential for the immune response and the target of several immunosuppresents, binds its targets. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474960907065-PUCQH7LN3UVEVFQSBY4T/PxIxIT+Library+2+CN+WT+250+nM.jpg MRBLEs Project Gallery A high-throughput assay for profiling protein-peptide interactions While advances in deep sequencing have dramatically improved our ability to profile how DNA and RNA binding proteins interact with their substrates, we lack similar tools for quantitative, high-throughput investigation of protein-peptide interactions. We have recently demonstrated that peptides can be synthesized directly on our encoded MRBLEs, laying the foundation for novel high-throughput on-bead assays to probe how proteins recognize their peptide substrates. In collaboration with the Cyert Lab, we are using this platform to understand how calcineurin, a human phosphatase essential for the immune response and the target of several immunosuppresents, binds its targets. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474960526618-2AJQNJY4FV0LY172J8PR/dmd_light_directed_synthesis_CROP.png MRBLEs Project Gallery A cheap and scalable way to synthesize custom peptide libraries on encoded beads The ability to flexible construct specific peptide libraries on spectrally encoded beads would provide a powerful proteomic tool. Using a commercial video projector ($300, Amazon.com), we are developing a new system that uses patterned light to synthesize specific peptide sequences onto beads spatially arrayed within a microfluidic device. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474960538778-3WYXH6GDGDQBZZL1S89L/Composite+of+img0000s.png MRBLEs Project Gallery Using encoded beads to link genotype and phenotype for many single cells in parallel Recent developments in single-cell analysis have enabled the probing of phenotypes, transcriptomes, and genomes for individual cells, revealing previously hidden heterogeneity within cell populations of high significance for human health and disease. The next great challenge is to understand both the cause and effect of this heterogeneity by linking changes in genetic information with their functional effects. We are developing a new technologies that uses oligonucleotide arrays coupled to encoding beads to link different types of measurements for the same cell. http://www.fordycelab.com/publications-gallery-2014 daily 0.75 2016-09-27 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474992852121-XLI8IIL7V13UU9ISV3QN/LysNetworkDiagram_Small.jpg Publications Gallery 2014 How duplicated transcription regulators can diversify to govern the expression of non overlapping sets of genes. Perez, J.C., Fordyce, P.M., Lohse, M.B., Hanson-Smith, V., DeRisi, J.L., & Johnson, A.D. Genes & Development (2014). (pdf) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474992852121-XLI8IIL7V13UU9ISV3QN/LysNetworkDiagram_Small.jpg Publications Gallery 2014 How duplicated transcription regulators can diversify to govern the expression of non overlapping sets of genes. Perez, J.C., Fordyce, P.M., Lohse, M.B., Hanson-Smith, V., DeRisi, J.L., & Johnson, A.D. Genes & Development (2014). (pdf) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474993034967-9DSFEYSC8MHCRKZ9M7VF/larva02.JPG Publications Gallery 2014 Hydraulic expulsion of tumbu fly larvae. Dybbro, E., Fordyce, P., Ponte, M., & Arron, S.T. JAMA Derm. (2014). (pdf) (web) http://www.fordycelab.com/publications-gallery-2013 daily 0.75 2016-09-27 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474993380123-OHI06THKBY6FK27RHG7A/WhiteOpaqueSwitching_Small.jpg Publications Gallery 2013 Structure of the transcriptional network controlling white-opaque switching in Candida albicans. Hernday, A.D., Lohse, M.B.*, Fordyce, P.M.*, Nobile, C.J., DeRisi, J.L., & Johnson, A.D. Molecular Microbiology (2013). (* denotes equal authorship). (pdf) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474993380123-OHI06THKBY6FK27RHG7A/WhiteOpaqueSwitching_Small.jpg Publications Gallery 2013 Structure of the transcriptional network controlling white-opaque switching in Candida albicans. Hernday, A.D., Lohse, M.B.*, Fordyce, P.M.*, Nobile, C.J., DeRisi, J.L., & Johnson, A.D. Molecular Microbiology (2013). (* denotes equal authorship). (pdf) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474993527561-4ABSESFQHQI67F8FFKS7/FOXP2Motif_HumanOnly.jpg Publications Gallery 2013 Microfluidic affinity and ChIP-Seq analyses converge on a conserved FOXP2-binding motif in chimp and human, which enables the detection of evolutionarily novel targets. Nelson, C.S., Fuller, C.K., Fordyce, P.M., Greninger, A.L., Li, H., & DeRisi, J.L. Nucleic Acids Research (2013). (pdf) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474993688964-BDGP4JY6BWGK3RC9W6H9/FinalWor3Motif.jpg Publications Gallery 2013 Identification and characterization of a previously undescribed family of sequence-specific DNA-binding domains. Lohse, M.B., Hernday, A.D., Fordyce, P.M., Noiman, L., Sorrells, T.R., Hanson-Smith, V., Nobile, C., DeRisi, J.L., & Johnson, A.D. PNAS (2013). (pdf) (web) http://www.fordycelab.com/publications-gallery-2012 daily 0.75 2016-09-27 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474995136135-KFWYA24OT3TAQ2TQWYLH/Hac1Motifs_150PH.png Publications Gallery 2012 Basic leucine zipper transcription factor Hac1 binds DNA in two distinct modes as revealed by microfluidic analyses. Fordyce, P.M., Pincus, D., Kimmig, P., Nelson, C.S., El-Samad, H., Walter, P., & DeRisi, J.L. PNAS (2012). (pdf) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474995136135-KFWYA24OT3TAQ2TQWYLH/Hac1Motifs_150PH.png Publications Gallery 2012 Basic leucine zipper transcription factor Hac1 binds DNA in two distinct modes as revealed by microfluidic analyses. Fordyce, P.M., Pincus, D., Kimmig, P., Nelson, C.S., El-Samad, H., Walter, P., & DeRisi, J.L. PNAS (2012). (pdf) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474995177657-OWXG1UQ3MPPQWDKLLLGA/BFBeadsInSerpentine_10x_0.7x_small.jpg Publications Gallery 2012 Programmable microfluidic synthesis of spectrally encoded microspheres. Gerver, R.E.*, Gomez-Sjoberg, R.*, Baxter, B.C.*, Thorn, K.S.*, Fordyce, P.M.*, Diaz-Botia, C.A., Helms, B.A., & DeRisi, J.L. Lab on a Chip (2012). (* denotes equal authorship, author order was determined by random draw). (pdf) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474995837894-94PL5C4S29H79OVKP1FY/Microfluidics-5031.jpg Publications Gallery 2012 Systematic characterization of feature dimensions and closing pressures for microfluidic valves produced via photoresist reflow. Fordyce, P.M., Diaz-Botia, C.A., DeRisi, J.L., & Gomez-Sjoberg, R. Lab on a Chip (2012). (pdf) (web) http://www.fordycelab.com/publications-gallery-2001 daily 0.75 2016-09-27 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1475017412154-VUM68T1PT0PP3ZOESODB/ktev_logo.gif Publications Gallery 2001 Search for the decay KL -> pi(0)e+e-. Alavi-Harati, A., et al. Physical Review Letters (2001). (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1475017412154-VUM68T1PT0PP3ZOESODB/ktev_logo.gif Publications Gallery 2001 Search for the decay KL -> pi(0)e+e-. Alavi-Harati, A., et al. Physical Review Letters (2001). (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1475017499355-E6K7K7DGZG5D50N2RX91/ktev_logo.gif Publications Gallery 2001 Measurement of the branching ratio of the decay KL -> e+e-gammagamma. Alavi-Harati, et al. Physical Review D (2001). (web) http://www.fordycelab.com/publications-gallery-2003 daily 0.75 2016-09-27 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1475016646012-B5HOJPMB4PHLZKZI1NB5/JBiolForceAndFluorescence.jpg Publications Gallery 2003 Combined optical trapping and single-molecule fluorescence. Lang, M.J., Fordyce, P.M., & Block, S.M. Journal of Biology (2003). (pdf) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1475016760145-FM13DZW7DLD3YXA3VCJE/KinesinRunLengths.jpg Publications Gallery 2003 Stepping and stretching: how kinesis uses external strain to step processively. Rosenfeld, S.S., Fordyce, P.M., Jefferson, G.M., King, P.H., & Block, S.M. Journal of Biological Chemistry (2003). (pdf) (web) http://www.fordycelab.com/publications-gallery-2011 daily 0.75 2016-09-27 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474996368638-GQ4709CIXVL7003M1G0V/EMBLImage.jpg Publications Gallery 2011 Integrating systems biology data to yield functional genomics insights. Fordyce, P., & Ingolia, N. Genome Biology (2011). (pdf) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1474996368638-GQ4709CIXVL7003M1G0V/EMBLImage.jpg Publications Gallery 2011 Integrating systems biology data to yield functional genomics insights. Fordyce, P., & Ingolia, N. Genome Biology (2011). (pdf) (web) http://www.fordycelab.com/publications-gallery-2008 daily 0.75 2016-09-27 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1475006567703-1QFDY6WA4CSLJFSQL3FB/SurfacePhotos.jpg Publications Gallery 2008 Advances in surface-based assays for single molecules. Fordyce, P.M.*, Valentine, M.T.*, & Block, S.M. in "ingle-Molecule Techniques: A Laboratory Manual" (Cold Spring Harbor Monograph Series) (2008). (* denotes equal authorship) (pdf) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1475006567703-1QFDY6WA4CSLJFSQL3FB/SurfacePhotos.jpg Publications Gallery 2008 Advances in surface-based assays for single molecules. Fordyce, P.M.*, Valentine, M.T.*, & Block, S.M. in "ingle-Molecule Techniques: A Laboratory Manual" (Cold Spring Harbor Monograph Series) (2008). (* denotes equal authorship) (pdf) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1475006520843-MQ4LKKY5X97SC8LVJFK6/SurfacePhotos.jpg Publications Gallery 2008 http://www.fordycelab.com/publications-gallery-2010 daily 0.75 2016-09-27 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1475005831534-AXE7CO3197X4Z0DRJCGE/devicefromend_wlightbox_01_cropped.jpg Publications Gallery 2010 De novo identification and biophysical characterization of transcription factor binding with microfluidic affinity analysis. Fordyce, P.M.*, Gerber, D.*, Tran, D., Zheng, J., Li, H., DeRisi, J.L., & Quake, S.R. Nature Biotechnology (2010). (* denotes equal authorship) (pdf) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1475005831534-AXE7CO3197X4Z0DRJCGE/devicefromend_wlightbox_01_cropped.jpg Publications Gallery 2010 De novo identification and biophysical characterization of transcription factor binding with microfluidic affinity analysis. Fordyce, P.M.*, Gerber, D.*, Tran, D., Zheng, J., Li, H., DeRisi, J.L., & Quake, S.R. Nature Biotechnology (2010). (* denotes equal authorship) (pdf) (web) http://www.fordycelab.com/publications-gallery-2004 daily 0.75 2016-09-27 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1475009623653-4XR3V48ZC28JXJ60NWYJ/OpticsDiagram.jpg Publications Gallery 2004 Simultaneous, coincident optical trapping and single-molecule fluorescence. Lang, M.J.*, Fordyce, P.M.*, Engh, A.M., Neuman, K.C., & Block, S.M. Nature Methods (2004). (* denotes equal authorship). (pdf) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1475009623653-4XR3V48ZC28JXJ60NWYJ/OpticsDiagram.jpg Publications Gallery 2004 Simultaneous, coincident optical trapping and single-molecule fluorescence. Lang, M.J.*, Fordyce, P.M.*, Engh, A.M., Neuman, K.C., & Block, S.M. Nature Methods (2004). (* denotes equal authorship). (pdf) (web) http://www.fordycelab.com/publications-gallery-2006 daily 0.75 2016-09-27 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1475007809624-W0JNLXQMZBH7U8OKAD9T/Eg5AssayFromCellDivision.jpg Publications Gallery 2006 Eg5 steps it up! Valentine, M.T., Fordyce, P.M., & Block, S.M. Cell Division (2006). (pdf) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1475007809624-W0JNLXQMZBH7U8OKAD9T/Eg5AssayFromCellDivision.jpg Publications Gallery 2006 Eg5 steps it up! Valentine, M.T., Fordyce, P.M., & Block, S.M. Cell Division (2006). (pdf) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1475008041594-0GIDTDA4LTBN3QH82LHA/Eg5SteppingForceClamp.jpg Publications Gallery 2006 Individual dimers of the mitotic kinetin Eg5 step processively and support substantial loads in vitro. Valentine, M.T.*, Fordyce, P.M.*, Krzysiak, T.C., Gilbert, S.P., & Block, S.M. Nature Cell Biology (2006). (* denotes equal authorship) (pdf) (web) http://www.fordycelab.com/publications-gallery-2017 daily 0.75 2021-01-27 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1507846103837-A2W11OWS99RN78X9NLGB/Screen+Shot+2017-10-12+at+3.08.09+PM.png Publications Gallery 2017 An open-Source, programmable pneumatic setup for operation and automated control of single-and multi-Layer microfluidic devices. Brower, K., Puccinelli, R., Markin, C., Shimko, T., Longwell, S., Cruz, B., Gomez-Sjoberg, R, Fordyce, P.; HardwareX (2017) (web); preprint on bioRXiv (2017) (web). OSF repository available here. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1507846103837-A2W11OWS99RN78X9NLGB/Screen+Shot+2017-10-12+at+3.08.09+PM.png Publications Gallery 2017 An open-Source, programmable pneumatic setup for operation and automated control of single-and multi-Layer microfluidic devices. Brower, K., Puccinelli, R., Markin, C., Shimko, T., Longwell, S., Cruz, B., Gomez-Sjoberg, R, Fordyce, P.; HardwareX (2017) (web); preprint on bioRXiv (2017) (web). OSF repository available here. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1496951558151-AMFTJGJ9378B4RZZ8VU9/SPIE+logo.png Publications Gallery 2017 Peptide library synthesis on spectrally encoded beads for multiplexed protein/peptide bioassays. Nguyen, H.Q., Brower, K., Harink, B., Baxter, B, Thorn, K., Fordyce, P. Proc. SPIE 10061, Microfluidics, BioMEMS, and Medical Microsystems XV, 100610Z (2017) (web). https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1496114094613-6NCWWVOU1EHWLQBCD3RZ/joker_illustration.png Publications Gallery 2017 Joker de Bruijn: Sequence libraries to cover all k-mers using joker characters. Ornenstein, Y., Puccinelli, R., Kim, R., Fordyce, P., and Berger, B.; Cell Systems (2017) (web). http://www.fordycelab.com/publications-gallery-2018 daily 0.75 2019-10-19 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1555103186057-DULUGYALXL94DAOW47EZ/Screen+Shot+2018-12-09+at+9.29.32+PM.png Publications Gallery 2018 Diversification of DNA binding specificities enabled SREBP transcription regulators to expand the repertoire of cellular functions that they govern in fungi. Toledo, V. D. O., Puccinelli, R., Fordyce, P.M., and Perez, J.C. PLoS Genetics (in press). (PDF) (Web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1555103186057-DULUGYALXL94DAOW47EZ/Screen+Shot+2018-12-09+at+9.29.32+PM.png Publications Gallery 2018 Diversification of DNA binding specificities enabled SREBP transcription regulators to expand the repertoire of cellular functions that they govern in fungi. Toledo, V. D. O., Puccinelli, R., Fordyce, P.M., and Perez, J.C. PLoS Genetics (in press). (PDF) (Web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1563570699477-PCT0TGNBGH01YX086INV/Capture.PNG Publications Gallery 2018 BET-seq: Binding energy topographies revealed by microfluidics and high-throughput sequencing. Aditham, A.K., Shimko, T.C., and Fordyce, P.M. Methods in Cell Biology - Microfluidics on a Molecular Scale (2018). (PDF) (web). https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1541050796546-MW2LNRN0SO9XEXF9Y539/Screen+Shot+2018-10-31+at+10.38.50+PM.png Publications Gallery 2018 Satb1 integrates DNA sequence, shape, motif density and torsional stress to differentially bind targets in nucleosome-dense regions. Ghosh, R.P., Shi, Q., Yang, L., Reddick, M.P., Nikitina, T., Zhurkin, V.B., Fordyce, P., Stasevich, T.J., Chang, H.Y., Greenleaf, W.J., and Liphardt, J.T., preprint on bioRXiv 10/2018; doi:10.1101/450262. (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1525224030823-BZC2BG44PALY67C2PIC2/Screen+Shot+2018-05-01+at+6.18.40+PM.png Publications Gallery 2018 High-throughput chromatin accessibility profiling at single-cell resolution. Mezger, A., Klemm, S., Mann, I., Brower, K., Mir, A., Bostick, M., Farmer, A., Fordyce, P., Linnarsson, S., & Greenleaf, W.; Nat. Comm. (2018) (pdf) (web) (bioRXiv doi: 10.1101/310284) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1525224228287-TJS88RIBK2UTLKZUXA0X/Screen+Shot+2018-05-01+at+6.23.10+PM.png Publications Gallery 2018 Discovering epistatic feature interactions from neural network models of regulatory DNA sequences. Greenside, P.G., Shimko, T., Fordyce, P., & Kundaje, A.; preprint on bioRXiv (04/17/2018); doi: 10.1101/302711 (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1525222814126-76BOIG8P3QUQYZJGICO3/f1pD.png Publications Gallery 2018 Comprehensive, high-resolution binding energy landscapes reveal context dependencies of transcription factor binding. Le, D.D., Shimko, T.C., Aditham, A.K., Keys, A.M., Orenstein, Y., and Fordyce, P.M. PNAS (3/27,8) (web) (pdf) (preprint in bioRXiv (9/26/17) https://static1.squarespace.com/static/53aa0662e4b0d4cbb80321fe/5ae90d5e70a6ad102dad9eda/5bda94e4c2241bcd3a01bf5b/1541051620741/ Publications Gallery 2018 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1555103186057-DULUGYALXL94DAOW47EZ/Screen+Shot+2018-12-09+at+9.29.32+PM.png Publications Gallery 2018 Diversification of DNA binding specificities enabled SREBP transcription regulators to expand the repertoire of cellular functions that they govern in fungi. del Olmo Toledo, V., Puccinelli, R., Fordyce, P.M., & Perez, J.C. PLoS Genetics (in press). https://static1.squarespace.com/static/53aa0662e4b0d4cbb80321fe/5ae90d5e70a6ad102dad9eda/5cb0fe01fa0d602643b99d55/1555103841002/ Publications Gallery 2018 BET-seq: Binding energy topographies revealed by microfluidics and high-throughput sequencing. Aditham, A.K., Shimko, T.C., and Fordyce, P.M. Methods in Cell Biology - Microfluidics on a Molecular Scale (2018). (web). http://www.fordycelab.com/publications-gallery-2019 daily 0.75 2021-01-27 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1571443304320-A3P8XTOX7177AMAA4UXO/Screen+Shot+2019-10-18+at+5.01.24+PM.png Publications Gallery 2019 micrIO: An open-source autosampler and fraction collector for automated microfluidic input-output. Longwell, S.A., & Fordyce, P.M. Lab on a Chip (2019). (PDF) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1571443304320-A3P8XTOX7177AMAA4UXO/Screen+Shot+2019-10-18+at+5.01.24+PM.png Publications Gallery 2019 micrIO: An open-source autosampler and fraction collector for automated microfluidic input-output. Longwell, S.A., & Fordyce, P.M. Lab on a Chip (2019). (PDF) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1571443935137-LPURF960O1BFYTTOW41E/Screen+Shot+2019-10-18+at+5.11.42+PM.png Publications Gallery 2019 Why do long zinc finger proteins have short motifs? Zuo, Z., Billings, T., Walker, M., Petkov, P., Fordyce, P., & Stormo, G.D. bioRXiv (2019). (PDF) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1563555967327-4AGM29WUWEH6HWXAD595/Capture.PNG Publications Gallery 2019 Quantitative mapping of protein-peptide affinity landscapes using spectrally encoded beads. Nguyen, H. Q., Roy, J., Harink, B., Damle, N. P., Latorraca, N. R., Baxter, B. C., Brower, K., Longwell, S., Kortemme, T., Thorn, K. S., Cyert, M. S., Fordyce, P.M eLife 8 (2019): e40499 (PDF) (Web) (OSF repository with all data) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1563555941776-RWK5KAXH3I85QD5RPS8C/Capture.jpg Publications Gallery 2019 Live imaging of Aiptasia larvae, a model system for coral and anemone bleaching, using a simple microfluidic device Treuren, W. V.*, Brower, K. K*., Labanieh, L., Hunt, D., Lensch, S., Cruz, B., Cartwright, H. N., Tran, C., Fordyce, P. M. Scientific reports 9 (2019). (PDF) (Web) https://static1.squarespace.com/static/53aa0662e4b0d4cbb80321fe/5c75ae1253450a175ca1dd94/5c75ae2315fcc0721df3f649/1551216163921/ Publications Gallery 2019 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1563556027171-11MA1ASOFSC947PA4YHQ/Capture.PNG Publications Gallery 2019 An open-source software analysis package for Microspheres with Ratiometric Barcode Lanthanide Encoding (MRBLEs) Harink, B., Nguyen, H., Thorn, K., Fordyce, P. M. PLOS/ONE (PDF) (Web) (OSF Repository). http://www.fordycelab.com/dropception-projects-gallery daily 0.75 2020-03-17 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1574145728146-ETW89ZYSFM1UMUU9BGG6/DECharacterization.png Dropception Projects Gallery Systematic characterization of DE droplets – By studying correlations between flow rates, droplet size, and device geometries, we are systematically optimizing DE droplet generation and testing fundamental droplet fluid physics and surfactant compatibility in complex interfaces. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1574145728146-ETW89ZYSFM1UMUU9BGG6/DECharacterization.png Dropception Projects Gallery Systematic characterization of DE droplets – By studying correlations between flow rates, droplet size, and device geometries, we are systematically optimizing DE droplet generation and testing fundamental droplet fluid physics and surfactant compatibility in complex interfaces. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1584410966443-34HA3UB0YML16HI132N7/SingleCellMultiOmics.png Dropception Projects Gallery Multi-parameter single-cell analysis – Using sortable double emulsions, we are developing assays to simultaneously screen for rare cell populations and characterize downstream single cell gene expression (RNA-Seq) or epigenomic signatures (ATAC-Seq). The goal of these efforts is to enable new droplet-based techniques that allow tandem ‘omics measurements on the same single cell. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1574145744826-20RIV3KI8H9KWSBHN606/PlantCells.png Dropception Projects Gallery Characterization of individual plant cells at scale – The flexibility of double emulsion droplets permits us to bring single-cell analysis tools to new organisms and new assays. We are developing new ways to measure the transcriptomic profiles and phenotypes of individual rare plant cells of interest. http://www.fordycelab.com/mrbles-project-gallery-2019 daily 0.75 2020-01-29 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1574147344290-C9LB81HPAWUQ7AZ6C055/MRBLE-path.png MRBLEs Project Gallery 2019 MRBLE-path - In sepsis, the time to antibiotic treatment is critical for patient survival, and the choice of treatment depends on the pathogen responsible for infection. We are developing a new method to rapidly screen for the presence of multiple bacteria species in a single reaction using oligonucleotide hybridization probes conjugated to MRBLEs with a 1:1 relationship between probe sequence and embedded code, allowing multiplexed measurement of pathogens via imaging alone. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1574147344290-C9LB81HPAWUQ7AZ6C055/MRBLE-path.png MRBLEs Project Gallery 2019 MRBLE-path - In sepsis, the time to antibiotic treatment is critical for patient survival, and the choice of treatment depends on the pathogen responsible for infection. We are developing a new method to rapidly screen for the presence of multiple bacteria species in a single reaction using oligonucleotide hybridization probes conjugated to MRBLEs with a 1:1 relationship between probe sequence and embedded code, allowing multiplexed measurement of pathogens via imaging alone. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1574148631026-2LTNA1VDYLFKG3ZRJNKI/Screen%2BShot%2B2019-11-18%2Bat%2B11.28.23%2BPM.jpg MRBLEs Project Gallery 2019 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1574148690705-M1ST7EHAD4VVPKS2QR8A/MRBLEPepHeatMap.png MRBLEs Project Gallery 2019 MRBLE-pep - While advances in deep sequencing have dramatically improved our ability to profile how DNA and RNA binding proteins interact with their substrates, we lack similar tools for quantitative, high-throughput investigation of protein-peptide interactions. Here, we synthesize libraries of peptides directly on MRBLEs with a 1:1 linkage between embedded code and peptide sequence, allowing high-throughput measurement of 100 weak protein-peptide interactions in parallel using very little material. In collaboration with the Cyert lab, we are using this platform to understand how human signaling proteins recognize their downstream targets. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1574147535101-M4KSF0EZSN6VYGHYJZF3/BATTLES.png MRBLEs Project Gallery 2019 BATTLES - T cells can discriminate between self- and non-self-antigens with exquisite sensitivity and specificity: even a single non-self antigen in a sea of 100,000 self antigens. Despite the central importance of this process for immune surveillance, we do not yet understand the mechanisms involved or how to manipulate them to generate new therapies. We are using MRBLEs to allow high-throughput measurements of how T cell activation depends on presented antigen sequence and applied forces. http://www.fordycelab.com/mitomi-2019 daily 0.75 2019-11-20 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1574281611840-G6FGHOQ8N4ILFUZ1TQTI/CACGTG.jpg MITOMI 2019 Probing the dark matter of regulatory DNA - Though > 90% of disease-causing variants in the human genome occur outside protein-coding regions, how these non-coding variants impact levels of gene expression remains largely unknown. To address this gap, we are leveraging the MITOMI platform to probe how sequence context outside transcription factor binding sites tunes binding kinetics and thermodynamics. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1574281611840-G6FGHOQ8N4ILFUZ1TQTI/CACGTG.jpg MITOMI 2019 Probing the dark matter of regulatory DNA - Though > 90% of disease-causing variants in the human genome occur outside protein-coding regions, how these non-coding variants impact levels of gene expression remains largely unknown. To address this gap, we are leveraging the MITOMI platform to probe how sequence context outside transcription factor binding sites tunes binding kinetics and thermodynamics. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1574281660902-C59YY8GVJBAYZD91HAGC/TFMutations.jpg MITOMI 2019 Dissecting the functional effects of transcription factor mutations - Transcription Factor (TF) proteins bind regulatory DNA sequences to control the expression of downstream genes, and mutations to TFs can affect the affinity and specificity of DNA binding. We are using microfluidic devices to quantitatively measure DNA binding affinities and dissociation kinetics for 1000s of TF-DNA interactions in parallel. This approach provides critical experimental data needed to build computational models of effects of TF mutations required for precision medicine. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1574281688495-I04ZXD715HG9SNFNX6FQ/HT-MEK.jpg MITOMI 2019 HT-MEK: a new kind of enzymology - We have developed a new platform (HT-MEK, for high-throughput microfluidic enzyme kinetics) that enables, for the first time, the rapid and quantitative measurement of multiple parameters for designed enzyme libraries. To date, we have characterized over 1,100 enzyme mutants (measuring >10,000 individual kinetic and thermodynamic parameters), and have identified structurally segregated regions controlling distinct biochemical properties within a model system phosphatase. Ultimately, these measurements will help yield next-generation predictive models of mutational effects required for rational enzyme design and precision medicine. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1574281951115-U312ISGLMIZYP1JBGQ6O/HT-MES.jpg MITOMI 2019 High-throughput and quantitative measurements of protein stability - Disease-associated protein mutations often act by destabilizing protein structure. In personalized medicine, the majority of disease-associated mutations are of unknown significance, preventing clinical action. We are developing parallel assays to quantify folding stability for thousands of proteins variants in parallel, using next-generation sequencing and fluorescence microscopy. With these approaches, we aim to characterize the protein stability landscape and better understand mechanisms of loss-of-function involved in disease. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1574281929272-MVAD8K9T9HQVWE99U4JB/BET-seq.jpg MITOMI 2019 BET-seq: ultra-high-throughput measurement of transcription factor-DNA binding affinities - The rapidly decreasing costs of next-generation sequencing (NGS) present an opportunity to sequence and identify millions of DNA molecules in parallel. Here, we paired MITOMI devices with NGS to quantitatively measure transcription factor binding energies for a million sequences in a single experiment. We are currently applying this platform to study how flanking nucleotides outside the canonical binding site can impact TF-DNA binding affinity. http://www.fordycelab.com/research-summary-gallery-2019 daily 0.75 2020-03-17 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1574221655539-YBUBIIPUYAN3TWA2ZU04/devicefromend_wlightbox_01_cropped_SMALL.jpg Research Summary Gallery 2019 Array-based multiplexing experiments (MITOMI and HT-MEK) employ microfluidic devices containing 1,568 valved reaction chambers aligned to printed DNA arrays. We are currently using these devices to better understand how transcription factors find and bind their genomic targets to regulate gene expression, as well as to understand how enzymes achieve their extraordinary catalytic efficiency and substrate specificity. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1574221655539-YBUBIIPUYAN3TWA2ZU04/devicefromend_wlightbox_01_cropped_SMALL.jpg Research Summary Gallery 2019 Array-based multiplexing experiments (MITOMI and HT-MEK) employ microfluidic devices containing 1,568 valved reaction chambers aligned to printed DNA arrays. We are currently using these devices to better understand how transcription factors find and bind their genomic targets to regulate gene expression, as well as to understand how enzymes achieve their extraordinary catalytic efficiency and substrate specificity. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1574149041636-QH32F5IF9WOESJ9D6P19/CoverArt_Option2.jpg Research Summary Gallery 2019 MRBLEs (Microspheres with Ratiometric Barcode Lanthanide Encoding) rely on spectral multiplexing to track analytes throughout an experiment. We can create microspheres containing > 1,000 distinct ratios of lanthanide nanophosphors that can be uniquely identified via imaging alone, and are now using these MRBLEs in a variety of downstream assays. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1584410885816-YOMTSNNABPBUI5B46ERM/DEDroplets.jpg Research Summary Gallery 2019 Dropception is a microfluidic platform for creating double emulsion (water-in-oil-in-water) droplets that can be sorted in high-throughput using standard flow cytometers (FACS machines). We recently demonstrated the ability to generate and sort double emulsion droplets without breakage, isolate individual rare droplets of interest in wells of a multiwell plate, and recover all encapsulated nucleic acids, enabling a wide range of novel single-cell multi-omic techniques. http://www.fordycelab.com/microwells-project-gallery-2019 daily 0.75 2019-11-20 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1574224698379-JVB4XDOQGYNSVW8ATYWF/Screen+Shot+2019-11-19+at+8.31.09+PM.png Microwells Project Gallery 2019 Organoid models of cancer have revolutionized our ability to recapitulate and observe in vivo cancer processes in a controlled in vitro environment. We are collaborating with Christina Curtis and Calvin Kuo to develop a microwell platform for performing high-throughput phenotyping of organoid heterogeneity across tumor development. This platform allows us to perform time-lapse imaging of thousands of organoids isolated in microwells, followed by computational reconstruction of growth trajectories for each organoid. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1574224698379-JVB4XDOQGYNSVW8ATYWF/Screen+Shot+2019-11-19+at+8.31.09+PM.png Microwells Project Gallery 2019 Organoid models of cancer have revolutionized our ability to recapitulate and observe in vivo cancer processes in a controlled in vitro environment. We are collaborating with Christina Curtis and Calvin Kuo to develop a microwell platform for performing high-throughput phenotyping of organoid heterogeneity across tumor development. This platform allows us to perform time-lapse imaging of thousands of organoids isolated in microwells, followed by computational reconstruction of growth trajectories for each organoid. http://www.fordycelab.com/publications-gallery-2020 daily 0.75 2021-08-12 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1606773488823-PUUEW2RDWR3UXLMGRGCN/Screen+Shot+2020-11-30+at+1.57.49+PM.png Publications Gallery 2020 Revealing enzyme functional architecture via high-throughput microfluidic enzyme kinetics. Markin, C.J.*, Mokhtari, D.A.*, Sunden, F., Appel, M.J., Akiva, E., Longwell, S.A., Sabatti, C., Herschlag, D.‡, & Fordyce, P.M.‡, Science (in press). (web) (pdf) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1593543248615-X7ZDNM3NUS1T0DPR1TVR/Screen+Shot+2020-06-30+at+11.48.06+AM.png Publications Gallery 2020 MRBLES 2.0: High-throughput generation of chemically functionalized spectrally and magnetically-encoded hydrogel beads using a simple single-layer microfluidic device. Feng, Y., White, A.K., Hein, J.B., Appel, E.A., & Fordyce, P.M., Microsystems & Nanoengineering (2020). (web) (pdf) (OSF repository with all data and design files) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1594182135604-933PFQK9TWYTYU1UV0GU/image-asset.jpeg Publications Gallery 2020 High-throughput binding affinity measurements for mutations spanning a transcription factor-DNA interface reveal affinity and specificity determinants Aditham, A.K., Markin, C.J.*, Mokhtari, D.A.*, DelRosso, N.V., & Fordyce, P.M. Cell Systems (2020). (pdf) (web) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1594181421494-JSY3DF0988Q4O4K933VI/Screen+Shot+2020-07-07+at+9.09.52+PM.png Publications Gallery 2020 Double emulsion picoreactors for high-throughput single-cell encapsulation and phenotyping via FACS. Brower, K.B.*, Khariton, M.*, Suzuki, P.H., Still, C., Kim, G., Calhoun, S.G.K., Qi, L.S., Wang, B.*, & Fordyce, P.M.* Analytical Chemistry (2020). (web) (pdf) (OSF repository with all data and design files) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1588222370563-FT7J4CRFOXM3ON0HNQRM/Screen+Shot+2020-04-29+at+9.52.37+PM.png Publications Gallery 2020 Protocol for peptide synthesis on spectrally encoded beads for MRBLE-pep assays. Hein, J.B., Nguyen, H.Q., Cyert, M., & Fordyce, P.M. Bio-protocols (2020). https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1571443018570-2WLROYHPM4HXAFYY8H9F/Screen+Shot+2019-10-18+at+4.56.34+PM.png Publications Gallery 2020 Optimized double emulsion flow cytometry with high-throughput single-droplet isolation. Brower, K.B., Carswell-Crumpton, C., Klemm, S., Cruz, B., Kim, G., Calhoun, S.G.K., Nichols, L, & Fordyce, P.M. Lab on a Chip (2020). (PDF) (web) (OSF repository with all data and design files) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1588222544021-O3EQ8FZ4RTFL4AF2WMTO/Screen%2BShot%2B2019-10-18%2Bat%2B4.51.01%2BPM.jpg Publications Gallery 2020 DeCoDe: degenerate codon design for complete protein-coding DNA libraries. Shimko, T.C., Fordyce, P.M., & Orenstein, Y. Bioinformatics (2020). (PDF) (web) http://www.fordycelab.com/publications-gallery-2021 daily 0.75 2021-08-23 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1629739126729-AXMJY1GKIIK2L9D9O4NM/image-asset.png Publications Gallery 2021 uPIC-M: efficient and scalable preparation of clonal single mutant libraries for high-throughput protein biochemistry. Appel, M.J., Longwell, S.A., Morri, M., Neff, N., Herschlag, D., & Fordyce, P.M. bioRXiv (2021). (web) (pdf) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1629739126729-AXMJY1GKIIK2L9D9O4NM/image-asset.png Publications Gallery 2021 uPIC-M: efficient and scalable preparation of clonal single mutant libraries for high-throughput protein biochemistry. Appel, M.J., Longwell, S.A., Morri, M., Neff, N., Herschlag, D., & Fordyce, P.M. bioRXiv (2021). (web) (pdf) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1606773488823-PUUEW2RDWR3UXLMGRGCN/Screen+Shot+2020-11-30+at+1.57.49+PM.png Publications Gallery 2021 Revealing enzyme functional architecture via high-throughput microfluidic enzyme kinetics. Markin, C.J.*, Mokhtari, D.A.*, Sunden, F., Appel, M.J., Akiva, E., Longwell, S.A., Sabatti, C., Herschlag, D.‡, & Fordyce, P.M.‡, Science (2021). (web) (pdf) • Science Perspective by Baumer & Whitehead. • Discussion in Nature. • Stanford News article. • Chemical & Engineering News article. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1619644202657-QVKPGIFKEEETI9I72H3B/Screen+Shot+2021-04-28+at+2.09.51+PM.png Publications Gallery 2021 Structure-activity mapping of the peptide- and force-dependent landscape of T-cell activation Feng, Y., Zhao, X., White, A.K., Garcia, K.C., & Fordyce, P.M., bioRxiv (2021). (web) (pdf) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1619717115743-4B7NTD99R04KQYXROONT/Screen%2BShot%2B2021-04-29%2Bat%2B10.20.38%2BAM.jpg Publications Gallery 2021 Fundamentals to function: quantitative and scalable approaches for measuring protein stability. Atsavapranee, B.*, Stark, C.D.*, Sunden, S., Thompson, S.‡, & Fordyce, P.M.‡. Cell Systems (2021). (web) (pdf) https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1608267912266-33J4YSKY5JDXIRKXM4P3/Screen+Shot+2020-12-16+at+2.55.34+PM.png Publications Gallery 2021 MRBLE-pep measurements reveal accurate binding affinities for B56, a PP2A regulatory subunit. Hein, J.B., Cyert, M.S., & Fordyce, P.M. ACS Measurement Science (in press). (web) (pdf) http://www.fordycelab.com/publications daily 0.75 2015-02-14 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1403740741962-OG0T2R4JIXGZ62LZPS4R/Lys14NetworkStructure.jpg Publications https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1403902840631-58I9OI2F2R9D6JDLWT9I/TumbuFlyLarva.jpeg Publications https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1403742572158-5JD2RTHHINRIBV9M533D/WhiteOpaqueSwitching_Small.jpg Publications https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1403752767055-WAHA1S09QZRSQYDVO7AU/FOXP2_PSAMs Publications https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1403758158164-1UTEBFLVR0VE0BVCL1PS/FinalWOR3Motif.jpeg Publications https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1403759477991-WCCC9G3CLGEWLFQA9YGV/FullMotifComparison.jpeg Publications https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1403753828205-5PB8XSDZBJ258RU49NJV/FalseColorSerpentine.jpg Publications https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1403753979580-W2JMSX1R2OAVCHEMKJ9Z/AZ50ValveCloseup.jpeg Publications https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1404141683985-7IQY6B285IL8113EAE8X/EMBL.jpeg Publications https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1403889265645-H0OIU8TQA6CW0IDP0UTO/image-asset.png Publications https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1403889597412-T7TGDWKAX0YDYFU6ZE7O/image-asset.jpeg Publications https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1404140972358-75MCF5FGTGOTI64PILWJ/Eg5AssayCartoon.jpeg Publications https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1403889835711-KEN126I6FITSU94WJ70N/Eg5SteppingForceClamp.jpg Publications https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1404142814907-EFB21547B0TTVP0PJK8H/OpticsDiagram.jpeg Publications https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1404163524900-M00ZIWOFL03PKJUFWGCU/image-asset.jpeg Publications https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1404143838220-H4SVY4UBW9G4IH0TAE3E/KinesinRunLengths.jpeg Publications http://www.fordycelab.com/research daily 0.75 2014-08-09 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1404174175552-WDAC3EXKIX4QU167VQ9F/MITOMI2.0.jpeg Research https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1404175162513-XP8IZ31Q66BV6R32J25Q/BeadCodeClusters_24CodeSet.jpeg Research https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1404175343561-90QH48UCI4GJ94B6E0G7/BeadsInSerpentineBF.jpeg Research http://www.fordycelab.com/movies daily 0.75 2014-07-01 http://www.fordycelab.com/contact daily 0.75 2025-05-22 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1403909540867-B8FGRDGE2TASBTYF73JP/image-asset.jpeg Contact Image of Stanford University courtesy Wikipedia. http://www.fordycelab.com/microfluidic-design-files daily 0.75 2024-05-07 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1403893171774-IXTFQA1G4L3XCUCEYACB/PF4kDevice.jpg Microfluidic device design files https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1403894310837-227G9J3126TIV0R18OU2/PC1kPhoto.jpg Microfluidic device design files https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/fc05f60f-88a5-4222-90c2-4bdf17aafedf/PS4blk_inuse.jpg Microfluidic device design files - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1403986488833-YRV110BHVZV3KVN8ZWQW/SerpentineDevice.jpeg Microfluidic device design files https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1404165797835-G7H8K8Y1RAB5ER822OGM/AZ50XTValveTestDevice.jpeg Microfluidic device design files http://www.fordycelab.com/3d-printed-parts daily 0.75 2017-03-08 http://www.fordycelab.com/publications-1 daily 0.75 2021-04-28 http://www.fordycelab.com/logistics daily 0.75 2017-05-02 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1486519455441-VAHEQMGEXT4BIOLBZISW/Screen+Shot+2016-12-16+at+6.15.25+PM.png Logistics Fordyce Lab at Alway Medical Building, October 2016. http://www.fordycelab.com/welcome-packet daily 0.75 2017-02-08 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1486519315405-OGS7CBPQE49Y8SA4HG20/image-asset.png Welcome Packet Europium doped lanthanide nanoparticles under deep UV excitation. December 2016, KB. http://www.fordycelab.com/general-protocols daily 0.75 2017-04-27 http://www.fordycelab.com/purchasing-information daily 0.75 2018-12-11 http://www.fordycelab.com/publications-v2 daily 0.75 2026-02-25 http://www.fordycelab.com/teaching daily 0.75 2024-03-15 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/2950a90e-7605-4a2e-8c37-592f301d30d3/Cardio_1+%281%29.png Teaching - Make it stand out Co-encapsulation of double emulsion droplets (orange) and MRBLEs beads (blue) in single emulsions. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/e737e6b5-66b0-49cc-9bb8-9fb4bd4f35ef/Cardio_2.png Teaching - Make it stand out Encapsulated plant protoplasts engineered to constitutively express GFP from large Nicotiana benthamiana leaves in single emulsion droplets. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/f1977098-3da6-4aa3-8f69-6bf6838482eb/Wormz_1.png Teaching - Make it stand out Schematic of trapping mechanism. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/719ea744-652d-47d6-a0d5-54c67c91a11f/Wormz_2.png Teaching - Make it stand out C. elegans at end of 10 µm trapping region. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/467ed4f4-875d-4af0-9c53-ec1900e013b9/droplets_2.png Teaching - Make it stand out Double emulsions trapped in U-trap device. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/7d485c26-030d-4658-8da1-308c6b900f5e/droplets_1.png Teaching - Make it stand out Double emulsions trapped in microwells device. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/9acf252c-89ef-4c16-9091-5833ffeac104/poop1.png Teaching - Make it stand out Flow and shear stress simulations of single and dual biofilm growth chambers. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/780d99d9-768c-4013-9143-99306721ae11/poop2.png Teaching - Make it stand out Fluorescence signal immediately after the bacteria were injected (top, t = 0 hrs) and after overnight incubation (bottom, t = 24 hrs). https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/233ff0db-2ff8-498b-a73b-35afa9268221/dropletProduction.jpg Teaching - Make it stand out Encapsulating single B. braunii in water/oil droplets. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/329c26f5-3f48-49f7-a3ff-4968b1009e55/oilgaeinDoubleEmulsions.jpg Teaching - Make it stand out Growing B. braunii colonies within water/oil/water droplets. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/93410b84-45b0-4e05-b29d-1c5e213a0ff4/stainedDoubleEmulsions.jpg Teaching - Make it stand out BODIPY-stained water/oil/water droplets. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/c371f9ea-86bb-4843-9cea-325364f94e53/Screen+Shot+2022-10-26+at+3.34.37+PM.png Teaching - Make it stand out Photo showing device with generated gradient. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/d56cc8ae-6da0-4927-9faa-645bfc255c63/Screen+Shot+2022-10-26+at+3.36.19+PM.jpg Teaching - Make it stand out Composite image over 100 minutes showing stuck “sticker” caulobacter within the first chamber. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1bc0ee40-6e8b-49de-875e-63812fd25221/Screen+Shot+2022-10-26+at+3.36.26+PM.png Teaching - Make it stand out Composite image over 1.5 seconds showing highly motile “swimmer” caulobacter within the second chamber. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/20439711-0484-42dc-9bf1-faff0ad7d408/Screen+Shot+2022-10-26+at+3.48.07+PM.png Teaching - Make it stand out Photomask designs for design iteration Round 1 and 2. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/28603444-c08f-422f-8a00-ef4b421bf382/waferForHaystack.jpg Teaching - Make it stand out Image of fabricated wafer with 15 µm separations between pillars. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/4c189ca9-1d10-49f8-bf28-57669438da44/experimentalSetupHaystack.png Teaching - Make it stand out Image of microscope with fabricated device and tubing. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/35f67c7f-a004-4877-8b81-5ded1258b4aa/Screen+Shot+2022-10-26+at+4.01.24+PM.png Teaching - Make it stand out Round 1: Main channel of cross-flow filtration device showing a large target cell remaining in the channel while a smaller cell gets pushed through pillars and lysed. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/7bd6dc54-e20d-49ad-944d-67618895b79e/Screen+Shot+2022-10-26+at+4.01.32+PM.png Teaching - Make it stand out Round 2: Target cells persisted in the main channel of the cross-flow filtration device without lysis after the between-pillar distance was reduced to 30 µm. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/6130b099-d728-486e-b24a-654bd6ed594b/Screen+Shot+2022-10-26+at+4.32.46+PM.jpg Teaching - Make it stand out Bonded glass device surrounding a thin membrane to facilitate co-culture. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/e669c7d2-1dec-48d5-a158-4ff56755c3a0/Screen+Shot+2022-10-26+at+4.33.27+PM.png Teaching - Make it stand out Human umbilical vein cells (HUVECs) growing on one side of the filter cell membrane within the glass device. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/c92c824d-2939-4953-a55f-5819324354f8/Screen+Shot+2022-10-26+at+5.10.51+PM.jpg Teaching - Make it stand out Image of red blood cells passing through small tapered channels to assess deformability. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/e488a55c-192f-4e9d-b71d-a3d19711882a/Screen+Shot+2022-10-26+at+4.56.59+PM.png Teaching - Make it stand out Example design for tapered channel trap devices. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/063abf96-67a7-4bf1-a61c-2c654a95ba4f/Screen+Shot+2022-10-26+at+4.54.15+PM.jpg Teaching - Make it stand out L2 worm immobilized in a 15 µm tall channel tapered from 20 µm to 5µm with an 0.5 mm extension. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/3e3b538f-586d-4a77-8b60-56e90a0a4f58/Screen+Shot+2022-10-26+at+4.54.24+PM.jpg Teaching - Make it stand out L2 worm immobilized to allow tracking of fluorescently-labeled sex myoblast cells over 8 hours. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1571085534675-SJ880W0WJD2UOY9C9ZAY/Screen+Shot+2019-10-14+at+1.28.58+PM.png Teaching ‘Horseshoe device’ cell loading: Day 1. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1571086614028-OPL0Z1AYW91ZJN0G4XH9/Screen+Shot+2019-10-14+at+1.49.12+PM.png Teaching Pneumatic and imaging setup for hydrodynamic breadboard. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1571165852476-DBSRAIM8K4N8ZCNM58LL/Screen+Shot+2019-10-15+at+11.56.53+AM.png Teaching Image of small (5 µm) features at device nozzle. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1571165899653-BQU1L0WP55HY3E3WKGSX/Screen+Shot+2019-10-14+at+5.36.48+PM.png Teaching Polydisperse double emulsion droplets with sizes ranging from 2 µm to 30 µm. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1571093262500-WH3Z8R8NCR72T24E43ED/image-asset.png Teaching Bright field image of 2 layer flow-focusing device. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1571093279209-12RX4LKI2IS15G9CTHVF/image-asset.png Teaching Bright field image showing co-axial flows. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1541574827057-5UCX4CCFO2V8WVETIVY4/Screen+Shot+2018-11-06+at+11.13.16+PM.png Teaching Figure 1. Schematic showing microfluidic planarian guillotines with varying channel widths after the guillotine blade. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1541576966112-M6JM0ZREPTH2F7O5F6UX/Screen+Shot+2018-11-06+at+11.48.49+PM.png Teaching Figure 1. Device design schematic. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1541576585005-301QOTX0OKLE96BHZ3OC/image33.png Teaching Figure 1. Fluorescence image showing macrophages (green) and E. coli (red) within a chamber of the microfluidic device. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1541623795423-6AJOZEWFMG3TEO7U1N36/Screen+Shot+2018-11-07+at+12.49.28+PM.png Teaching Figure 1. Microfluidic droplet generator connected to syringe pumps on microscope illumination stage. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1541623732761-ZZ3T9P0106HD1KYP8VIV/Screen+Shot+2018-11-07+at+12.48.12+PM.png Teaching Figure 2. Droplets produced via T-junction (top) and flow focusing (bottom) devices at different flow rates. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1541571724965-0N1Z2TZHE6CTRP9I897D/Screen+Shot+2018-11-06+at+10.20.15+PM.png Teaching Figure 1 . Shear stress modeling results from COMSOL and particle image velocimetry. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1541571974125-0UWFLIBFA02BVSY0HNMP/Screen+Shot+2018-11-06+at+10.25.11+PM.png Teaching Figure 2. Example images showing overnight growth of cells. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1541573752617-YA7NLLM6DXHZL6I8VMQY/Screen+Shot+2018-11-06+at+10.31.37+PM.png Teaching FIgure 1. Device schematic. (A) Each separation chamber has different pillar spacings to capture cells of different sizes. (B) Integrated valves allow selective retrieval of particular cell sizes. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1541625611589-9RY3B6EAIVUU3CTMO1Z1/Screen+Shot+2018-11-07+at+1.19.41+PM.png Teaching Figure 2. Image of the microfluidic device containing pillars with 50 µm gaps between them and 50 µm diameter beads successfully captured under constant pressure flow. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/1541573442219-QFEOCWBXP0WXRSXJ0GKE/Screen+Shot+2018-11-06+at+10.42.48+PM.png Teaching Figure 1. Image showing array of traps with Aiptasia larvae within them. http://www.fordycelab.com/research-2019 daily 0.75 2025-04-29 http://www.fordycelab.com/people_new daily 0.75 2026-01-13 http://www.fordycelab.com/publication-data daily 0.75 2021-05-19 http://www.fordycelab.com/foundry daily 0.75 2023-07-25 https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/244cb490-7614-48a2-99ad-2f03751793c0/logofilled.png Foundry - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/057832a9-1942-421d-a155-ebcf98d5a6df/Outreach1.png Foundry - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/c3719105-d00f-4790-a003-6f08f89df8cc/Cleanroom2.png Foundry - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/e4a31ca1-efa6-4bf5-8655-886d9fb7c3d2/wormsdevice.jpg Foundry - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/53aa0662e4b0d4cbb80321fe/dab5cc73-47fd-4b9f-8d78-33e8223e3a56/Image+from+iOS+%282%29.jpg Foundry - Make it stand out Whatever it is, the way you tell your story online can make all the difference. http://www.fordycelab.com/service-leadership daily 0.75 2023-11-15 http://www.fordycelab.com/new-page-1 daily 0.75 2026-03-22 http://www.fordycelab.com/data daily 0.75 2026-03-23