The Fordyce Lab

MITOMI Protocols

All of Robert's collected protocols can be found in a single document here.

In addition, all of these protocols are available individually:

1. Preparing fluorescently-labeled dsDNA libraries for relative measurement of affinities at a single concentration.
2. Preparing fluorescently-labeled dsDNA libraries for measuring concentration-dependent binding (absolute affinities).
3. Spotting DNA arrays with DeRisi arrayer.
4. Spotting DNA arrays with Quake arrayer (deprecated).
5. Evaluating and running MITOMI devices.
6. MITOMI imaging suggestions.
7. MITOMI gridded image acquisition.
8. MITOMI affinity measurement image acquisition (single equilibrium measurement).
9. MITOMI dissociation rate measurement image acquisition (multiple dissociation measurements).
10. Preparing and acquiring flat-field correction images.
11. Applying flat-field correction images.
12. Stitching gridded acquisition images.
13. Image analysis with MITOMIAnalysis matlab code (available on Fordyce lab github).
14. Acquiring Cy5 intensity calibration curves (to relate measured intensities to concentrations).